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CRISPR Gene Editing Now Possible in Cockroaches - SciTechDaily

CRISPR Gene Editing Now Possible in Cockroaches - SciTechDaily

CRISPR Gene Editing Now Possible in Cockroaches - SciTechDaily
May 17, 2022 1 min, 20 secs

According to a paper published in the journal Cell Reports Methods by Cell Press on May 16th, 2022, researchers devised a CRISPR-Cas9 technique to enable gene editing in cockroaches.

“By improving the DIPA-CRISPR method and making it even more efficient and versatile, we may be able to enable genome editing in almost all of the more than 1.5 million species of insects, opening up a future in which we can fully utilize the amazing biological functions of insects.” — Takaaki Daimon.

Current approaches for insect gene editing typically require microinjection of materials into early embryos, severely limiting its application to many species.

In addition, insect gene editing often requires expensive equipment, a specific experimental setup for each species, and highly skilled laboratory personnel.

“These problems with conventional methods have plagued researchers who wish to perform genome editing on a wide variety of insect species,” Daimon says.

The successful application of DIPA-CRISPR in two evolutionarily distant species demonstrates its potential for broad use.

Despite these limitations, DIPA-CRISPR is accessible, highly practical, and could be readily implemented in laboratories, extending the application of gene editing to a wide diversity of model and non-model insect species.

“By improving the DIPA-CRISPR method and making it even more efficient and versatile, we may be able to enable genome editing in almost all of the more than 1.5 million species of insects, opening up a future in which we can fully utilize the amazing biological functions of insects,” Daimon says.

Reference: “DIPA-CRISPR is a simple and accessible method for insect gene editing” by Yu Shirai, Maria-Dolors Piulachs, Xavier Belles and Takaaki Daimon, 16 May 2022, Cell Reports Methods.

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