Dutchwoman Anna van der Breggen won the women's time trial after American favourite Chloe Dygert crashed out at the Road World Championships in Italy.
Dygert, a 10-time world champion on track and road, was 26 seconds ahead on the clock at the intermediate checkpoint.
Van der Breggen, who secured her third Giro Rosa title on Saturday, came second to Dygert in last year's world time trial in Yorkshire.
The 30-year-old was also runner-up to compatriot Annemiek van Vleuten as she tried to defend the road race title she won in 2018 - her only previous world gold?